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Oligonucleotides used as <t> PCR </t> primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening
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Oligonucleotides used as <t> PCR </t> primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening
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Oligonucleotides used as <t> PCR </t> primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening
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Oligonucleotides used as <t> PCR </t> primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening
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Oligonucleotides used as <t> PCR </t> primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening
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Oligonucleotides used as <t> PCR </t> primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening
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Image Search Results


Oligonucleotides used as PCR primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening

Journal: Hereditas

Article Title: Mutations in the gene of the Gα subunit of the heterotrimeric G protein are the cause for the brachytic1 semi-dwarf phenotype in barley and applicable for practical breeding

doi: 10.1186/s41065-017-0045-1

Figure Lengend Snippet: Oligonucleotides used as PCR primers to amplify and sequence the gene encoding the Gα-subunit of the heterotrimeric G protein and to amplify gene fragments located in the genomic region surrounding this gene in a BAC pool screening

Article Snippet: PCR amplification from genomic DNA and sequencing of PCR products was done at LGC Genomics GmbH (Berlin, Germany) in the case of all brh1 original mutant lines, near-isogenic lines and the respective mother cultivars.

Techniques: Sequencing, Amplification